Method of targeting dna

Details Method of targeting dna Method of targeting dna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 9/22 (2006.01) C12N 15/11 (2006.01) C12N 15/90 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2095624

A method of forming a three-stranded DNA wherein each strand of the three-stranded DNA molecule is hybridized (that is, non-covalently bound) to at least one other strand of the three-stranded DNA molecule. The method comprises: contacting a recombination protein with a double-stranded DNA molecule and with a single-stranded DNA molecule sufficiently complementary to one strand of the double-stranded DNA molecule to hybridize therewith, which contacting is effected under conditions such that the single-stranded DNA molecule hybridizes to the double-stranded DNA molecule so that the three-stranded DNA molecule is formed. The method of forming a three-stranded DNA molecule can be used to specifically cleave a target DNA sequence, or to protect a double-stranded DNA molecule from modification.

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