Methods for treating or preventing angiogenesis-dependent...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61P 9/10 (2006.01)

Patent

CA 2507534

A method for enhancing the transfection efficiency of naked plasmid DNA in treating and/or preventing angiogenesis-dependent symptoms is provided by the present inventions. According to the present method, a suitable naked plasmid DNA is subjected for intramuscular injection under increased pressure inside the muscle or hyperbaric oxygen. Angiogenesis-dependent symptoms, including wounds, inflammatory diseases, critical limb ischemia, ischemic heart diseases, cerebral infarction, diabetic neuropathy, spinal canal stenosis, etc., may be treated by the present methods.

L'invention concerne une méthode permettant d'accroître l'efficacité de transfection d'un ADN plasmidique nu dans le traitement et/ou la prévention des symptômes dépendant de l'angiogénèse. Selon la méthode, un ADN plasmidique nu approprié est injecté par voie intramusculaire dans le muscle sous une pression accrue ou conjointement avec de l'oxygène hyperbarique. Des symptômes dépendant de l'angiogénèse, notamment des blessures, des maladies inflammatoires, l'ischémie critique du membre inférieur, des maladies cardiaques ischémiques, l'infarctus cérébral, la neuropathie diabétique, la sténose du canal rachidien, etc., peuvent être traités au moyen des méthodes selon l'invention.

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