Method of providing a substrate with a ready-to-use,...

Details Method of providing a substrate with a ready-to-use,... Method of providing a substrate with a ready-to-use,...

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2388723

Method of providing a substrate with a ready-to-use uniformly distributed extracellular matrix is disclosed. This method includes applying extracellular matrix components to a substrate area; incubating the extracellular matrix components to allow polymerization thereof; freezing the polymerized extracellular matrix on the substrate area; lyophilizing the frozen extracellular matrix on the substrate area; and allowing the lyophilized extracellular matrix to warm to room temperature. Also contemplated is a cell culture apparatus having a dried uniformly distributed extracellular matrix formed by the above- mentioned method.

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