Process for the staining of sperm

Details Process for the staining of sperm Process for the staining of sperm

A - Human Necessities

61

K

A61K 35/52 (2006.01) A61D 19/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2519989

Sperm cells are stained according to processes that involve the combining of sperm cells with a fluorescent DNA selective dye at an elevated temperature in excess of about 40~C. The methods allow for a decreased staining time. The cells may thereafter be efficiently sorted according to common separation methods, including flow cytometry.

L'invention concerne des procédés permettant de colorer les cellules du sperme et consistant à combiner celles-ci avec un colorant sélectif de l'ADN fluorescent à une température élevée supérieure à environ 40 ·C. Les procédés permettent d'obtenir un temps de coloration réduit. Les cellules peuvent ensuite être triées efficacement selon des procédés de séparation commune, notamment la cytométrie en flux.

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