Enzymatic assay kit and method applicable to whole cells

Details Enzymatic assay kit and method applicable to whole cells Enzymatic assay kit and method applicable to whole cells

G - Physics

01

N

167/44

G01N 33/573 (2006.01) C12Q 1/28 (2006.01) G01N 33/551 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2012934

IN THE CANADIAN PATENT AND TRADEMARK OFFICE PATENT APPLICATION entitled: Enzymatic assay kit and method applicable to whole cells in the name of: Dominique CARRIERE Assignee: SANOFI ABSTRACT OF THE DISCLOSURE The present invention relates to a kit for assay- ing the endogenous cell enzymes characteristic of a cell population or subpopulation, said kit comprising the following components: a) a solid support to which one or more mono- clonal antibodies are fixed by covalent bonding or by physical adsorption, said monoclonal antibodies being directed against surface antigens of the cell population to be assayed; and b) one or more solutions providing the reagents (substrate and chromogen) necessary for developing the activity of the endogenous enzyme to be assayed It further relates to an enzymatic method of assaying the endogenous enzymes of a cell population or subpopulation. The assay kit and the enzymatic method according to the invention can be used for assaying the myelo- peroxidase in the granulocytes of blood or urine.

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