Modified tetracycline repressor protein compositions and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) C07K 14/195 (2006.01) C07K 14/21 (2006.01) C07K 14/245 (2006.01) C07K 16/12 (2006.01)

Patent

CA 2471333

The present invention relates to a system for regulating gene expression in prokaryotes using modified tetracycline repressor proteins. In particular, the present invention relates to modified tetracycline repressor proteins that exhibit a "reverse" phenotype in prokaryotic organisms, nucleic acids encoding these repressor proteins, methods for identifying and preparing these proteins, and methods for using these proteins for regulating gene expression in prokaryotic organisms, in drug screening assays and for identifying non- antibiotic compounds that are specific inducers of these modified repressor proteins.

Cette invention concerne un système permettant de réguler l'expression des gènes dans des procaryotes au moyen de répresseurs de tétracycline modifiés. Cette invention porte en particulier sur des répresseurs de tétracycline modifiés qui présentent un phénotype "inverse" dans des organismes procaryotiques, sur des acides nucléiques qui codent ces répresseurs, sur des méthodes d'identification et de préparation de ces protéines répresseurs ainsi que sur des méthodes d'utilisation de ces protéines pour la régulation de l'expression des gènes dans des organismes procaryotiques, pour les tests de criblage de médicaments et pour l'identification de composés non antibiotiques constituant des inducteurs spécifiques de ces répresseurs modifiés.

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