Proteome epitope tags and methods of use thereof in protein...

Details Proteome epitope tags and methods of use thereof in protein... Proteome epitope tags and methods of use thereof in protein...

G - Physics

01

N

G01N 33/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2545899

Disclosed are methods for reliably detecting the presence of proteins, especially proteins with various post~-translational modifications (phosphorylation, glycosylation, methylation, acetylation, etc.) in a sample by the use of one or more capture agents that recognize and interact with recognition sequences uniquely characteristic of a set of proteins (Proteome Epitope Tags, or PETs) in the sample. Arrays comprising these capture agents or PETs are also provided.

L'invention concerne des méthodes permettant de détecter fiablement la présence de protéines, notamment, de protéines pourvues de diverses modifications post-translationnelles (phosphorylation, glycosylation, méthylation, acétylation, etc.) dans un échantillon au moyen d'au moins un agent de capture qui permet de reconnaître et d'interagir avec des séquences de reconnaissance qui sont uniquement caractéristiques d'une ensemble de protéines (étiquettes d'épitope de protéome ou PET) dans l'échantillon. Cette invention a aussi pour objet des jeux ordonnés d'échantillons comportant ces agents de capture ou PET.

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