C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/14 (2006.01) C07K 1/00 (2006.01) G01N 33/02 (2006.01) G01N 33/10 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2447085
The invention relates to a method for extracting the gluten contained in a sample consisting in using an ethanol aqueous solution in the presence of a composition containing a disulphur group reducer and a decoupling agent, in a buffer having a pH of between 7 and 8. The inventive method can be used to extract quantitatively the gluten contained in a heat-processed or non-heat- processed food sample before the quantification of the gluten by ELISA. Said method is suitable for food analyses, in particular, for foodstuffs intended for coeliac sufferers.
La présente invention concerne un procédé destiné à l'extraction du gluten contenu dans un échantillon, consistant à utiliser une solution aqueuse d'éthanol en présence d'une composition renfermant un agent réducteur de groupes disulfure et un agent de dissociation, dans une solution tampon ayant un pH compris entre 7 et 8. Ce procédé permet d'extraire quantitativement le gluten contenu dans un échantillon d'aliment ayant subi ou non un traitement thermique, avant la quantification du gluten par dosage immunoenzymatique (ELISA). Ce procédé s'applique à l'analyse d'aliments et en particulier à l'analyse d'aliments destinés aux malades coeliaques.
Llorente Gomez Mercedes
Lopez Villar Elena
Mendez Corman Enrique
Consejo Superior de Investigaciones Cientificas
Fetherstonhaugh & Co.
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