Primer for nucleic acid detection

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C40B 30/00 (2006.01) C40B 30/04 (2006.01) C40B 40/06 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2594223

This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5'-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable signal from the label when the universal primer hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using the universal primer in nucleic acid amplification, such as real-time PCR.

L'invention concerne des amorces universelles marquées utilisées pour la détection et l'amplification de molécules d'acides nucléiques. Ces amorces universelles peuvent être reliées à l'extrémité 5' d'une amorce spécifique de séquence cible. Dans des exemples particuliers, ladite amorce universelle comprend un nucléotide marqué flanqué sur les deux côtés d'un nucléotide dont les nucléotides complémentaires modifient un signal détectable à partir de l'indicateur lorsque ladite amorce universelle est hybridée avec sa molécule d'acide nucléique complémentaire. L'invention concerne également des procédés d'utilisation de ladite amorce universelle dans l'amplification d'acide nucléique, par exemple la PCR en temps réel.

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