Modified polymerases and attenuated viruses and methods of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A61K 39/13 (2006.01) A61K 39/145 (2006.01) A61K 39/21 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2673535

Compositions and methods involving modified viral polymerases with one or more substitutions of different amino acids at conserved regions of the polymerase result in enzymes with varying rates and fidelity of replication A universally applicable polymerase mechanism based strategy for the production of attenuated viruses and anti-viral vaccines is also disclosed

L'invention concerne des compositions et des procédés se rapportant à des polymérases virales ayant une ou plusieurs substitutions de différents acides aminés à des régions conservées de la polymérase et fournit des enzymes avec des vitesses et une fidélité de réplication variable. L'invention concerne une stratégie à base d'un mécanisme de polymérase, applicable de façon universelle, pour la fabrication de virus atténués et de vaccins antiviraux.

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