Vaccines with an ltb adjuvant

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/39 (2006.01) A61K 39/145 (2006.01) C07K 14/11 (2006.01) C07K 14/245 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2311492

The present invention relates to a vaccine containing at least one particulate immunogen and an adjuvanting amount of B subunits of heat-labile enterotoxin characteristic of E. Coli. More in particular, this invention relates to vaccines wherein the adjuvanting LTB is free from contaminating A subunits or holotoxin. To this end, preferably, use is made of LTB prepared by recombinant DNA techniques. The particulate immunogens can relate to or can be derived from e.g. viruses, bacteria or fungi. This vaccine is particularly suitable for the induction of a protective response against said particulate immunogen upon mucosal (e.g. intra-nasal) administration. It was found that such administration results in both systemic and mucosal protection against the pathogen to which the particulate immunogen relates.

L'invention porte sur un vaccin comportant au moins un immunogène particulaire et une quantité additionnelle de la sous-unités B de l'entérotoxine thermolabile, caractéristique du E.coli. Elle porte plus particulièrement sur des vaccins dont le LTB additionnel est exempt de sous-unités A ou d'holotoxines contaminantes. On utilise de préférence à cet effet du LTB obtenu par recombinaison d'ADN. L'immunogène particulaire peut par exemple être apparenté à des virus, des bactéries ou des champignons ou en dériver. Ledit vaccin est particulièrement indiqué pour susciter une réponse protectrice contre l'immunogène particulaire lorsqu'il est appliqué sur les muqueuses (par exemple par voie intra-nasale). On a découvert qu'une telle administration assure à la fois une protection systémique et des muqueuses contre l'élément pathogène apparenté à l'immunogène particulaire.

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