Continuous directed evolution of proteins and nucleic acids

Details Continuous directed evolution of proteins and nucleic acids Continuous directed evolution of proteins and nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2738635

The present invention discloses generalizable methods of evolving nucleic acids and proteins utilizing continuous directed evolution. The invention discloses methods of passing a nucleic acid from cell to cell in a desired function-dependent manner. The linkage of the desired function and passage of the nucleic acid from cell to cell allows for continuous selection and mutation of the nucleic acid.

La présente invention concerne des procédés généralisables pour faire évoluer des acides nucléiques et des protéines au moyen d'une évolution dirigée continue. L'invention concerne des procédés de passage d'un acide nucléique d'une cellule à une autre d'une manière dépendant d'une fonction désirée. La liaison de la fonction désirée et du passage de l'acide nucléique d'une cellule à une autre permet la sélection et la mutation continues de l'acide nucléique.

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