C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/70 (2006.01) C12N 5/10 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2075488
2075488 9112337 PCTABS00006 A method provided for assaying for human immunodeficiency virus comprises: seeding dishes with cells susceptible to HIV infection; adding to the dishes a sample to be tested for HIV infection; incubating the cells; exposing the cells to a first antibody reactive with HIV proteins; exposing the cells to marker-conjugated antibody reactive with the first antibody; examining the dishes with a suitable microscope; and counting the number of foci of marker-positive cells per dish to determine the number of infected cells in the dish. The process can be used to detect and to quantify HIV-infected cells, which permits monitoring of the effects of drug therapy on the levels of HIV expression in AIDS patients, as well as providing a quantitative measure of HIV expression in asymptomatic HIV antibody-positive individuals.
Chesebro Bruce Wilcox
Wehrly Kathryn Ann
Gowling Lafleur Henderson Llp
U.s. Departm Ent Of Commerce The United States Of America Represented By The Secretary
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