Expression of an active carrier for xylose in genetically...

Details Expression of an active carrier for xylose in genetically... Expression of an active carrier for xylose in genetically...

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C07K 14/40 (2006.01) C12N 1/21 (2006.01) C12N 15/81 (2006.01) C12P 7/06 (2006.01)

Patent

CA 2618273

The present invention confers to the fermentative yeast Saccharomyces cerevisiae, genetically modified by insertion of a nucleic acid sequence encoding a xylose and glucose active transporter, the ability to assimilate xylose using a system of co-transport with protons exhibiting a high affinity for xylose. The invention is useful for the production of bioethanol from plant biomass and other lignocellulosic materials, using genetically modified microorganisms for assimilating and fermenting xylose in mixtures of hexoses and pentoses resulting from raw material of industrial interest.

L'invention confère à la levure de fermentation Saccharomyces cerevisiae, génétiquement modifiée par l'insertion d'une séquence d'acide nucléique codant pour un transporteur actif du xylose et du glucose, la capacité d'assimiler le xylose au moyen d'un système de cotransport comportant des protons présentant une grande affinité pour le xylose. L'invention est utile dans la production de bioéthanol à partir de biomasse végétale et d'autres matières lignocellulosiques, au moyen de micro-organismes génétiquement modifiés qui assimilent et fermentent le xylose dans des mélanges d'hexoses et de pentoses, et permet d'obtenir une matière première intéressante pour l'industrie.

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