Method for determination of glycoprotein ibalpha (gpibalpha)...

G - Physics – 01 – N

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G01N 33/86 (2006.01)

Patent

CA 2603732

The invention provides a novel assay system for detecting the presence, concentration and binding activity of GPIb.alpha. in a biological sample. The method for determining the presence of GPIb.alpha. in a biological sample comprises: (a) providing a substance comprising GPIb.alpha.; (b) contacting the substance from step (a) with a binding protein which binds to GPIb.alpha.; (c) adding a detection compound specific to GPIb.alpha.; (d) adding a complexing compound that binds the binding protein from step (b); and (e) detecting the detection compound from step (c) wherein a positive detection signal indicates the presence of GPIb.alpha. in the biological sample.

L'invention concerne un nouveau système d'analyse pour détecter la présence, la concentration et l'activité de liaison de la protéine GPIba dans un échantillon biologique. Le procédé de détermination de la présence de la protéine GPIba dans un échantillon biologique consiste : (a) à prendre une substance comprenant la protéine GPIba ; (b) à mettre en contact la substance de l'étape (a) avec une protéine de liaison qui se lie à la protéine GPIba ; (c) à ajouter un composé de détection spécifique de la protéine GPIba ; (d) à ajouter un composé complexant qui se lie à la protéine de liaison de l'étape (b) ; et (e) à détecter le composé de détection de l'étape (c), un signal de détection positif indiquant la présence de la protéine GPIba dans l'échantillon biologique.

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