Protease variants and compositions

C - Chemistry – Metallurgy – 12 – N

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C12N 15/57 (2006.01) C11D 3/386 (2006.01) C12N 9/54 (2006.01) C12N 9/56 (2006.01)

Patent

CA 2219949

A subtilisin having improved storage stability or improved performance in detergents, wherein one or more amino acid residues situated in, or in the vicinity of a hydrophobic domain of the parent subtilase have been substituted for an amino acid residue more hydrophobic than the original residue, said hydrophobic domain comprising the residues corresponding to residues P129, P131, I165, Y167, Y171 or BLS309 (in BASBPN numbering), and said residues in the vicinity thereof comprises residues corresponding to the residues E136, G159, S164, R170, A194, and G195 of BLS309 (in BASBPN numbering), with the exception of the R170M, R170I and R170Y variant of BLS309.

L'invention se rapporte à une subtilisine dont on améliore la stabilité au stockage et le rendement dans les détergents. Un ou plusieurs résidus d'acides aminés de ladite substance, situés à l'intérieur ou au voisinage d'un domaine hydrophobe de la subtilase parentale, ont été remplacés par un résidu d'acide aminé plus hydrophobe que le résidu initial. Le domaine hydrophobe considéré renferme les résidus correspondants aux résidus P129, P131, I165, Y167, Y171 de BLS309 ( en numérotation BASBPN), et les résidus situés au voisinage du domaine en question comprennent ceux qui correspondent aux résidus E136, G159, S164, R170, A194 et G195 de BLS309 (en numérotation BASBPN), à l'exception des variantes R170M, R170I et R170Y de BLS309.

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