Method for producing a peptide with a pi above 8 or below 5

Details Method for producing a peptide with a pi above 8 or below 5 Method for producing a peptide with a pi above 8 or below 5

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/18 (2006.01) C07K 1/36 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2334160

A method is described for producing a peptide having a pI above 8 or below 5 wherein the peptide is expressed as a fusion protein in which it is linked to a fusion partner through an acid cleavage site. The peptide is released from the fusion partner by acid cleavage in the absence of chaotrope. The fusion partner and its acid cleavage products, if any, have a net charge sufficiently different from that of the desired peptide to allow isolation of the peptide by ion-exchange chromatography.

L'invention concerne une méthode permettant de produire un peptide dont le pI est supérieur à 8 ou inférieur à 5, ce peptide étant exprimé sous la forme d'une protéine de fusion liée à un partenaire de fusion par l'intermédiaire d'un site de clivage acide. Ce peptide est libéré de ce partenaire de fusion par un clivage acide, en l'absence de tout chaotrope. Le partenaire de fusion, et le cas échéant, ses produits de clivage acide, présentent une charge nette suffisamment différente de celle du peptide souhaité pour permettre un isolement dudit peptide par chromatographie d'échange d'ions.

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