Production and purification of recombinant human...

Details Production and purification of recombinant human... Production and purification of recombinant human...

C - Chemistry, Metallurgy

12

N

C12N 15/24 (2006.01) A61K 38/20 (2006.01) C07K 1/16 (2006.01) C07K 14/54 (2006.01) C12N 15/63 (2006.01) C12N 15/75 (2006.01) C12N 15/86 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2028827

Abstract of the Invention Recombinantly produced IL-3 is purified in a series of steps, initially employing hydrophobic interaction, followed by ion-exchange chromatography and gel filtration. Furthermore methods are provided for improved production of recombinant hIL -3 either in glycosylated form from mammalian and yeast cells or in unglycosylated form from prokaryotes.

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