A nucleic acid diagnostic method using riboprobes, rnase...

Details A nucleic acid diagnostic method using riboprobes, rnase... A nucleic acid diagnostic method using riboprobes, rnase...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2182194

This invention is drawn to improved methods for detecting particular nucleic acid sequences via nucleic acid hybridization. Disclosed is a diagnostic method wherein detectably labeled riboprobes are hybridized in solution to complementary nucleic acid sequences encided by target nucleic acid molecules. Following hybridization, RNAse digestion is used to degrade unhybridized probe molecules, after which chromatographic separation is employed to isolate probe-target hybrids. This method improves the detection limit of nucleic acid hybridization and is generally useful in medical diagnostics, forensics, and molecular biology research.

Méthode améliorée de détection de séquences particulières d'acide nucléique par hybridation de l'acide nucléique et méthode de diagnostic où des ribosondes détectables par marquage sont hybridées en solution à des séquences complémentaires d'acide nucléique codées par des molécules cibles d'acide nucléique. Après l'hybridation, la digestion de la RNAse sert à dégrader les molécules sondes non hybridées après quoi on recourt à la séparation chromatographique pour isoler les hybrides de sondes-cibles. Cette méthode améliore les limites de détection de l'hybridation de l'acide nucléique et présente une utilité générale en matière de diagnostic médical, de chimie légale et de recherche en biologie moléculaire.

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