Improved ligand binding assays for vanilloid receptors

Details Improved ligand binding assays for vanilloid receptors Improved ligand binding assays for vanilloid receptors

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/536 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2425961

The present invention describes an assay in which pH alters RTX affinity to VR1 receptors. The RTX affinity was increased with increasing pH from 4.1 to 8.6. Both the RTX affinity and apparent number of RTX binding sites were decreased with increasing pH from 8.6 to 10.6. The high pH may be causing the cell membranes to denature. These pH conditions (pH 8.0 to pH 9.0) provide a high signal to noise ratio, give a more robust assay and require use of less experimental materials.

La présente invention concerne un dosage doté d'un pH modifiant une affinité à la RTX pour des récepteurs VR1. L'affinité à la RTX est accrue au moyen d'une augmentation de pH de 4,1 à 8,6. A la fois l'affinité à la RTX et le nombre apparent des accepteurs de RTX diminuent lorsque le pH passe de 8,6 à 10,6. Un pH élevé peut provoquer une dénaturation des membranes de cellules. Ces conditions de pH (pH 8,0 à pH 9,0) permettent d'obtenir un rapport signal-bruit élevé, un dosage plus fort, et de réduire la quantité de matières expérimentales utilisées.

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