Glycan-specific analytical tools

Details Glycan-specific analytical tools Glycan-specific analytical tools

C - Chemistry, Metallurgy

12

N

C12N 9/24 (2006.01) G06F 19/12 (2011.01) C12N 9/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/34 (2006.01) C40B 30/00 (2006.01) C40B 50/06 (2006.01) G01N 33/53 (2006.01) C07K 9/00 (2006.01)

Patent

CA 2749588

Provided are lectenz molecules, which are mutated carbo-hydrate processing enzyme enzymes that are catalytically inactive and that have had their substrate affinity in-creased by at least 1.2 fold. Further provided are methods for making and methods of using such lectenz. Addi-tional mutated proteins following the lectenz approach are further provid-ed.

La présente invention concerne des molécules de lectenz qui sont des enzymes de traitement des hydrates de carbone ayant subi une mutation, catalytiquement inactives et dont laffinité au substrat a été multipliée au moins par 1,2. Linvention porte aussi sur des procédés de préparation et dutilisation de ces lectenz. Linvention a également trait à des protéines mutées supplémentaires selon l'approche adoptée à l'égard des lectenz.

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