Use of chick beta actin gene intron-1

Details Use of chick beta actin gene intron-1 Use of chick beta actin gene intron-1

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 15/11 (2006.01) C12N 15/67 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2676651

A method to use chick beta actin gene intron-1 or functional equivalent as a gene expression enhancer eiement or a gene expression "hot spot" sequence for constructing or reconstructing a mammalian expression vector for extremely high expression of recombinant proteins is disclosed. Composition of a set of extremely strong gene expression vectors is also disclosed.

L'invention concerne une méthode d'utilisation de l'intron-1 du gène de la bêta-actine du poulet, ou son équivalent fonctionnel, comme élément d'amplification de l'expression génétique ou séquence "point chaud" de l'expression génétique, pour construire ou reconstruire un vecteur d'expression mammalien en vue d'obtenir une expression extrêmement élevée de protéines recombinantes. L'invention concerne en outre une composition d'un ensemble de vecteurs d'expression génétique extrêmement puissants.

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