G - Physics – 01 – N
Patent
G - Physics
01
N
167/37, 150/9
G01N 30/02 (2006.01) G01N 30/88 (2006.01) G01N 33/49 (2006.01) G01N 33/68 (2006.01) G01N 33/72 (2006.01) G01N 30/34 (2006.01) G01N 30/62 (2006.01) G01N 30/74 (2006.01)
Patent
CA 2004627
ABSTRACT A method of analyzing a liquid sample is disclosed. The liquid contains both glycated and nonglycated protein fractions. A support is provided having complexible hydroxy- boryl moieties bound thereto. The support is brought in contact with the liquid sample and with a transport solution for the nonglycated protein fraction, causing said glycated protein fraction to complex with said bound moieties. The transport solution and nonglycated protein fraction are separated from the column as a first analyte. The support and complexed glycated proteins are then contacted with an eluant for separating the glycated proteins from the support. The eluant and the separated glycated proteins are separated from the column as a second analyte. The first and second analytes are spectrophotometrically analyzed using light of a desired wavelength. The transport solution and eluant are spectrophotometrically balanced such that, in the quantitative high pressure liquid chromatographic determination of glycated protein in a 0.5 µl. liquid test sample containing 6 to 8 g./dl. of total protein and a glycated fraction amounting to 12 - 15% of the total protein content of the test sample. The determination is carried out at said desired wavelength. The calculated signal to noise ratio is equal to or greater than about 20, where the signal is the height of the absorbance peak produced by the glycated fraction of the test sample, and the noise is the greatest distance of deviation observed when a blank sample of the transport solution is measured at said desired wavelength and at otherwise identical test conditions. Both the height and distance are measured relative to an idealized baseline established by the absorbance at the desired wavelength of the transport solution.
Noffsinger Jimmie K.
Rexroad James H. Jr.
Teeman Bonnie A.
Wawrzinek Claus-Peter
Gowling Lafleur Henderson Llp
Primus Corporation
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