Method of detecting interacting proteins

G - Physics – 01 – N

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G01N 33/566 (2006.01) C07K 1/13 (2006.01) C07K 17/00 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2373491

A method for detecting proteins under mutual interaction which comprises mixing a protein having a label for detection synthesized by a cell-free protein synthesis method with another protein having a modification for separation, or synthesizing a protein having a label for detection and another protein having a modification for separation in a single system by the cell- free protein synthesis method; separating a pair of proteins formed by the interaction between these proteins with the use of the modification for separation as described above; and distinguishing these proteins with the use of the label for detection as described above. A screening method using this method.

Cette méthode de détection de protéines en interaction mutuelle consiste à mélanger une protéine possédant une étiquette de détection, protéine synthétisée grâce à une technique de synthèse de protéine hors-cellule, à une autre protéine ayant une modification aux fins d'une séparation, ou bien à synthétiser dans un seul système une protéine possédant une étiquette de détection et une autre protéine ayant une modification aux fins d'une séparation et ce, à l'aide de la technique de synthèse de protéine hors-cellule. La méthode consiste ensuite à séparer deux protéines formées par l'interaction existant entre ces protéines à l'aide de la modification pour séparation susmentionnée et, enfin, à distinguer ces protéines à l'aide de l'étiquette de détection. L'invention porte également sur un criblage faisant appel à cette méthode.

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