Nucleic acid sequences encoding polyenoic fatty acid...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/90 (2006.01) A01H 3/00 (2006.01) C12N 5/10 (2006.01) C12N 15/61 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2379813

Nucleic acid sequences and methods are provided for producing conjugated fatty acids in host cells. Nucleic acid sequences are obtained from an algal sources which encode polyenoic fatty acid isomerase. The nucleic acid sequences can be used in expression constructs to direct the expression of the PFI sequences in host cells. Expression of PFI in transgenic host cells provides for the production of conjugated fatty acids in the host cell.

L'invention concerne des séquences d'acides nucléiques ainsi que des procédés permettant de produire des acides gras conjugués dans des cellules hôtes. Les séquences d'acides nucléiques sont obtenues à partir d'algues qui codent pour des d'acides gras polyénoïques isomérase. Lesdites séquences peuvent servir dans des constructions d'expression pour diriger l'expression des séquences PFI dans des cellules hôtes. L'expression de PFI dans des cellules hôtes transgéniques permet de produire des acides gras conjugués dans la cellule hôte.

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