Biotin operon

Details Biotin operon Biotin operon

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C12N 15/52 (2006.01) C12N 15/67 (2006.01) C12P 17/18 (2006.01)

Patent

CA 2078043

A DNA sequence of a high biotin operon-expression system usable for breeding a bacterium having excellent biotin productivity is provided. The invention relates to a DNA sequence of the biotin operon characterized by having at least one base pair of either a nucleotide sequence of the regulatory region of the biotin operon of Escherichia coli or a nucleotide sequence in the vicinity of the bioB initiating codon which is mutated in comparison with the wild type strain. Escherichia coli transformed with a recombinant plasmid carrying such a DNA sequence has high biotin productivity.

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