Regulated expression of heterologous genes in plants and...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 9/14 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2195095

The present invention relates to chimeric genes having (i) a DNA sequence encoding a product that is effective to reduce ethylene biosynthesis in fruit from the plant, and (ii) a promoter whose expression is induced during fruit ripening or by ethylene synthesis by the fruit, where the DNA sequence is heterologous to the promoter and the DNA sequence is operably linked to the promoter to enable expression of the product. The invention describes vectors and transgenic plants, vegetables, flowers and fruits carrying the chimeric gene, as well as methods related thereto.

La présente invention se rapporte à des gènes chimères possédant (i) une séquence d'ADN codant un produit efficace pour réduire la biosynthèse d'éthylène dans le fruit d'une plante, et (ii) un promoteur dont l'expression est induite lors du mûrissement du fruit ou par la synthèse d'éthylène effectuée par le fruit. La séquence d'ADN est hétérologue au promoteur et est liée fonctionnellement au promoteur pour permettre l'expression du produit. L'invention se rapporte également à des vecteurs et à des plantes transgéniques, des légumes, des fleurs et des fruits portant le gène chimère ainsi qu'aux procédés relatifs à ceux-ci.

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