C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/805 (2006.01) C07K 1/113 (2006.01) C07K 1/18 (2006.01) C07K 1/22 (2006.01)
Patent
CA 2203133
The present invention generally relates to methods for purifying hemoglobin solutions and to hemoglobin solutions obtained by the methods. In one aspect, such methods include removing contaminants in crude hemoglobin-containing lysates with heat treatment. In a further aspect, the present invention provides methods for producing substantially purified hemoglobin solutions using immobilized metal affinity chromatography, optionally following by anion exchange chromatography.
La présente invention se rapporte à des procédés de purification de solutions d'hémoglobine et aux solutions d'hémoglobine obtenues selon ces procédés. Selon un aspect de cette invention, ces procédés consistent à retirer des contaminants, par traitement thermique, de lysats bruts contenant de l'hémoglobine. Selon un autre aspect, la présente invention concerne des procédés de production de solutions d'hémoglobine pratiquement purifiées par chromatographie d'affinité de métaux immobilisés, éventuellement suivie d'une chromatographie d'échange d'anions.
Durfee Steven L.
Mathews Antony J.
Matthews Maura-Ann H.
Milne Erin E.
Neway Justinian O.
Baxter Biotech Technology S.a.r.l.
Smart & Biggar
Somatogen Inc.
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