Proteases from gram-positive organisms

Details Proteases from gram-positive organisms Proteases from gram-positive organisms

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) C11D 3/386 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/56 (2006.01) C12N 9/90 (2006.01)

Patent

CA 2296708

The present invention relates to the identification of novel cysteine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis cysteine proteases CP1, CP2 and CP3. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding CP1, CP2 or CP3. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a cysteine protease of the present invention.

La présente invention concerne l'identification de nouvelles cystéines protéases dans des germes Gram positif. La présente invention concerne également les séquences d'acide aminé et d'acide nucléique des cystéines protéases CP1, CP2 et CP3 identifiées dans le Bacillus subtilis. La présente invention concerne, en outre, des cellules hôtes présentant une mutation ou une délétion d'une partie ou de la totalité du gène codant CP1, CP2 et CP3. La présente invention concerne également des cellules hôtes comprenant un acide nucléique codant des protéines hétérologues désirées telles que des enzymes. La présente invention concerne, en outre, une composition de nettoyage comprenant une cystéine protéase.

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