Multiply-substituted protease variants

Details Multiply-substituted protease variants Multiply-substituted protease variants

C - Chemistry, Metallurgy

12

N

C12N 9/54 (2006.01) A23K 1/165 (2006.01) C11D 3/386 (2006.01) C12N 15/57 (2006.01)

Patent

CA 2618389

Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.

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