Process for the expression of a recombinant gene

Details Process for the expression of a recombinant gene Process for the expression of a recombinant gene

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/70 (2006.01) C12N 9/72 (2006.01) C12N 15/11 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2002516

The present invention provides a process for the expression of a recombinant gene, which contains AGA and/or AGG codons for arginine, in Escherichia coli after transformation with an expression vector which contains the recombinant gene, wherein the amount of t-RNA present in the E. coli cells which incorporates arginine and recognizes the codons AGG and AGA is increased to at least fivefold of the amount normally occurring in these cells. The present invention also provides new expression vectors and new expression auxiliary vectors.

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