Method for characterizing, in particular for quantifying,...

G - Physics – 01 – N

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G01N 33/50 (2006.01)

Patent

CA 2733181

The invention relates to a method for characterizing, in particular for quantifying, molecular marker(s) that are intracellularly absorbed from tissues by blood macrophages that are recirculated from the tissues into the circulatory system, wherein the following steps are carried out: application of an agent to whole blood, said agent inhibiting coagulation and/or agglomeration of whole blood; selecting and/or enriching and/or separating blood macrophages or leukocyte populations that contain blood macrophages from whole blood; perforating and/or lysing the selected blood macrophages or leukocyte populations containing the blood macrophages, optionally after previous permeabilization thereof; qualitatively and quantitatively determining non blood macrophage markers, namely molecular markers arising from tissue, after previously perforating and/or lysing the blood macrophages or leukocyte populations containing the blood macrophages, and an apparatus for carrying out the method.

L'invention concerne un procédé de caractérisation, en particulier de quantification, de marqueurs moléculaires absorbés de manière intracellulaire à partir des tissus par des macrophages du sang provenant des tissus et remis en circulation dans le flux sanguin. Ce procédé comprend les étapes suivantes : application au sang total d'un moyen inhibant le caillement et/ou la coagulation du sang total ; à partir du sang total, réalisation d'une sélection et/ou d'un enrichissement et/ou d'une séparation de macrophages sanguins ou de populations de leucocytes contenant des macrophages sanguins ; réalisation d'une perforation et/ou d'une lyse des macrophages sanguins ou des populations de leucocytes contenant des macrophages sanguins, éventuellement après une perméabilisation préalable ; exécution d'une détermination qualitative et quantitative de marqueurs n'appartenant pas aux macrophages sanguins, c'est-à-dire des marqueurs moléculaires provenant des tissus, après perforation et/ou lyse préalables des macrophages sanguins ou des populations de leucocytes contenant des macrophages sanguins. L'invention concerne en outre un agencement pour la mise en uvre du procédé.

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