Signal amplification using circular hairpin probes

Details Signal amplification using circular hairpin probes Signal amplification using circular hairpin probes

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2690043

The present invention provides methods for detecting a target nucleic acid using a circular dual-hairpin probe that is formed upon the presence of the target nucleic acid. The detection methods find use in detecting the presence of antibody-antigen complexes and for detecting the binding of a ligand to its binding partner. Kits and reaction mixtures for performing the present methods are also provided.

La présente invention concerne des procédés pour détecter un acide nucléique cible employant une sonde à double épingle à cheveux circulaire qui se forme en présence de l'acide nucléique cible. Les procédés trouvent leur utilisation dans la détection de la présence de complexes anticorps-antigène et dans la détection de la liaison d'un ligand à son partenaire de liaison. L'invention concerne également des nécessaires et des mélanges réactionnels pour mettre en uvre les présents procédés.

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