C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) C12M 3/04 (2006.01)
Patent
CA 2307832
A method for enhancing the mixing of a varicella-infected cell culture in a roller bottle by the introduction of controlled cross-sectional flow perturbations in roller bottle rotation is disclosed. The effectiveness of the method is demonstrated by achieving higher efficiency in cell culturing and virus propagation in roller bottles by introducing controlled cross-sectional flow perturbations during the process.
L'invention concerne un procédé permettant d'améliorer le mélange d'une culture cellulaire infectée par la varicelle dans une bouteille pour agitateur rotatif, ce procédé consistant à introduire de manière régulée des perturbations selon un écoulement transversal au cours du pivotement de ladite bouteille pour agitateur rotatif. L'efficacité de ce procédé se traduit par un rendement amélioré des mises en culture cellulaire et une meilleure propagation virale dans les bouteilles pour agitateur rotatif, grâce à l'introduction des perturbations susmentionnées au cours du processus.
Bramble Joye L.
Muzzio Fernando J.
Searles James A.
Merck & Co. Inc.
Merck Sharp & Dohme Corp.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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