Modification of xylanases to increase thermophilicity,...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/24 (2006.01) C12N 15/31 (2006.01) C12N 15/56 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2649852

A modified Family 11 xylanase enzyme comprising cysteine residues at positions 99 and 118 to form an intramolecular disulfide bond is provided. The modified xylanase is produced by substitution of an amino acid at position 99, 118 or both positions 99 and 118 with a cysteine to produce the intramolecular disulfide bond. Xylanases of the invention display improved thermophilicity, alkalophilicity or thermostability relative to wild-type xylanases. Such xylanases find use in a variety of applications in industry that require enzyme activities at temperatures and/or pH values above that of the native enzyme.

L'invention a trait à une enzyme xylanase de la famille 11 modifiée, qui contient des résidus cystéine aux positions 99 et 118 afin de former une liaison disulfure intramoléculaire. La xylanase modifiée est produite par la substitution d'un acide aminé à la position 99 ou 118 ou à la fois aux positions 99 et 118 par une cystéine, ce qui permet de produire ladite liaison disulfure intramoléculaire. Les xylanases selon l'invention présentent de meilleures propriétés de thermophilie, d'alcalophilie ou de thermostabilité que les xylanases du type sauvage. De telles xylanases conviennent à une variété d'applications industrielles faisant appel à des activités enzymatiques nécessitant des températures et/ou des valeurs de pH supérieures à celles des enzymes natives.

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