Method of making factor-dependent human b cell lines

Details Method of making factor-dependent human b cell lines Method of making factor-dependent human b cell lines

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2071886

2071886 9109115 PCTABS00005 A method is provided for establishing factor-dependent human B cell lines capable of secreting immunoglobulin of a desired specificity and capable of long term culturing. The method includes selecting a resting B cell having immunoglobulin of the desired specificity and culturing it in the presence of an agent capable of cross-linking its CD40 surface antigens. Long term culture requires the continued presence of the cross-linking agent. Preferably, the cross-linking agent is a monoclonal antibody specific for the CD40 antigen, presented by non-replicating mammalian cells expressing Fc.gamma.-RII.

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