Methods for producing proteins using hamster igf-1

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/62 (2006.01) A61K 38/27 (2006.01) A61P 3/06 (2006.01) A61P 19/08 (2006.01) A61P 21/00 (2006.01) C07K 16/26 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/18 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2598570

A polypeptide selected from the group consisting of (1) a polypeptide having the amino acid sequence represented by SEQ ID NO:1 and (2) a polypeptide having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 by substitution of the amino acid at the 3-position by another amino acid and having an insulin-like growth factor 1 (IGF-1) activity; a DNA encoding the above polypeptide; a vector containing the above DNA; a host cell containing the above vector; and a method of producing a recombinant protein by using the above polypeptide.

La présente invention a pour objet un polypeptide sélectionné au sein du groupe constitué par (1) un polypeptide de séquence d'acides aminés représentée par SEQ ID NO:1 et (2) un polypeptide de séquence d'acides aminés dérivée de la séquence d'acides aminés représentée par SEQ ID NO:1 par substitution de l'acide aminé en position 3 par un autre acide aminé, et qui présente une activité de type facteur de croissance 1 ressemblant à l'insuline (IGF-1). La présente invention a également pour objet un ADN codant pour ledit polypeptide, un vecteur contenant ledit ADN, une cellule-hôte contenant ledit vecteur, et une méthode de production d'une protéine recombinante par emploi dudit polypeptide.

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