Chromogenic substrate for esterase and immunoassay using same

G - Physics – 01 – N

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167/43

G01N 33/53 (2006.01) C12Q 1/44 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2000678

P-1372 CHROMOGENIC SUBSTRATE FOR ESTERASE AND IMMUNOASSAY USING SAME ABSTRACT OF THE DISCLOSURE A chromogenic substrate. for an esterase, 4-[(3,5-dichloro-4-butyryloxyphenyl)imino]-2,5-cyclo- hexadien-l-one, ; is useful in an assay for determination of the presence or concentration of an enzyme or a ligand in a liquid. The assay may be an ELISA using the substrate or a dual enzyme assay including a blocked inhibitor which is cleaved by a hydrolase to an inhibitor of the esterase. The invention includes a kit of materials including the substrate for performing an assay.

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