Soybean glutathione-s-transferase enzymes

Details Soybean glutathione-s-transferase enzymes Soybean glutathione-s-transferase enzymes

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2340791

This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of soybean glutathione-S-transferase (GST) enzymes involved in the detoxification of xenobiotic compounds in plants and seeds. The invention also relates to the construction of chimeric genes encoding all or a substantial portion of soybean GST enzymes, host cells transformed with those genes and methods for the recombinant production of soybean GST enzymes. Methods of constructing transgenic plants having altered levels of GST enzymes and screens for identifying soybean GST enzyme substrates and soybean GST enzyme inhibitors are also provided.

Cette invention concerne des fragments d'acides nucléiques isolés codant la totalité ou une grande partie des enzymes de glutathione-S-transférase (GST) qui sont issues du soja et qui jouent un rôle dans la détoxification de composés xénobiotiques dans les plantes et les graines. Cette invention concerne également une structure de gènes chimériques qui codent la totalité ou une grande partie des enzymes de GST issues du soja, des cellules hôtes transformées à l'aide de ces gènes ainsi que des procédés de production recombinante d'enzymes de GST issues du soja. Cette invention concerne en outre des procédés de construction de plantes transgéniques possédant des niveaux modifiés d'enzymes de GST, ainsi que des cribles permettant d'identifier des substrats d'enzymes de GST issues du soja et des inhibiteurs d'enzymes de GST issues du soja.

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