Kindling fluorescent proteins and methods for their use

Details Kindling fluorescent proteins and methods for their use Kindling fluorescent proteins and methods for their use

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 1/00 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2447690

Kindling fluorescent protein compositions and nucleic acids encoding the same, as well as methods for using the same, are provided. The kindling fluorescent proteins are characterized in that they become brightly fluorescent proteins, from an initial non-fluorescent or low fluorescent state, upon exposure to a kindling stimulus, which fluorescent state may be reversible or irreversible. The subject protein/nucleic acid compositions find use in labeling protocols, e.g., in labeling proteins, organelles, cells and organisms, etc., in a variety of different types of applications. Also provided are systems and kits for use in practicing such applications.

L'invention concerne des compositions de protéines fluorescentes d'embrasement, des acides nucléiques codant lesdites protéines, ainsi que des procédés d'utilisation desdites compositions. Les protéines fluorescentes d'embrasement selon l'invention sont caractérisées en ce qu'elles deviennent fortement fluorescentes à partir d'un état initial de fluorescence nulle ou faible sous l'effet d'un stimulus d'embrasement, ledit état de fluorescence étant réversible ou irréversible. Les compositions de protéines/acides nucléiques selon l'invention peuvent être employées dans des protocoles de marquage, par ex. dans le marquage de protéines, d'organites, de cellules et d'organismes etc. dans diverses applications. L'invention concerne également des systèmes et des ensembles destinés à la mise en oeuvre desdites applications.

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