Polynucleotide synthesis using a processing enzyme

Details Polynucleotide synthesis using a processing enzyme Polynucleotide synthesis using a processing enzyme

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12N 13/00 (2006.01) C12N 15/10 (2006.01) C12N 9/12 (2006.01) C12N 11/02 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2367269

A method for polynucleotide synthesis, comprises the steps of: (i) reacting a polynucleotide processive enzyme, e.g. a polymerase or TdT, with a nucleotide substrate under conditions suitable for enzyme activity; and (ii) modulating the conformation of the enzyme, e.g. using radiation, to allow incorporation of a predetermined nucleotide.

L'invention concerne un procédé pour la synthèse de polynucléotides, consistant : (i) à faire réagir une enzyme de maturation polynucléotidique telle qu'une polymérase ou une transférase-terminale (TdT), avec un substrat nucléotidique aux conditions convenant à l'activité enzymatique; et (ii) à moduler la conformation d'une enzyme, au moyen notamment de radiations, pour permettre l'incorporation d'un nucléotide prédéterminé.

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