In vitro maintenance of hematopoietic stem cells

Details In vitro maintenance of hematopoietic stem cells In vitro maintenance of hematopoietic stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/0789 (2010.01) C12N 5/0775 (2010.01)

Patent

CA 2329519

The present invention is directed to human mesenchymal stem cells isolated from a tissue specimen, such as marrow cells, and to the method of co- culturing isolated mesenchymal stem cells and/or mesenchymal stem cell-derived adipocytes with hematopoietic progenitor cells such that the hematopoietic stem cells retain their phenotype.

La présente invention concerne des cellules souches mésenchymateuses humaines isolées d'un prélèvement tissulaire, telles que des cellules médullaires. L'invention concerne également un procédé de coculture de cellules souches mésenchymateuses isolées et/ou d'adipocytes issus de cellules souches mésenchymateuses avec des cellules précurseurs hématopoïétiques, ledit procédé de coculture permettant aux cellules souches mésenchymateuses hématopoïétiques de conserver le phénotype des cellules ayant fait l'objet de la coculture.

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