Culture for expanding stem cells ex-vivo

Details Culture for expanding stem cells ex-vivo Culture for expanding stem cells ex-vivo

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01)

Patent

CA 2693420

The present invention relates to a culture including a growth medium and a combination of cytokines consisting of i) interleukin-6 (IL6); ii) flt3-ligand (FLT3); iii) stem cell factor (SCF) and iv) thrombopoi- etin (TPO); the use of the culture for expanding.ex vivo stem cells and/or parental cells and cells differentiated therefrom, and the use of said cells obtainable from said expansion.

L'invention concerne une culture contenant un milieu de croissance et une combinaison de cytokines constituée i) d'interleukine-6 (IL6) ; ii) de ligand Flt3 (FLT3) ; iii) de facteur des cellules souches (FCS) ; et iv) de thrombopoïétine (TPO). L'invention concerne également l'utilisation de cette culture dans l'expansion ex vivo de cellules souches et/ou de cellules parentales et de cellules différenciées à partir de celles-ci, ainsi que l'utilisation des cellules obtenues à partir de cette expansion.

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