Expression vector and methods of producing high levels of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/79 (2006.01) C07K 14/505 (2006.01) C07K 14/715 (2006.01)

Patent

CA 2611946

A process for high expression of protein of interest using an expression vector. The process comprises at least the following regulatory elements: a) a CMV promoter, or its functional variants, b) an intron, c) TPL or its functional variants, d) VA genes or functional variants, and e) a bovine growth hormone polyadenylation sequence or functional variants.

L'invention concerne un procédé de niveaux d'expression élevés d'une protéine à analyser au moyen d'un vecteur d'expression. Ce procédé comprend au moins les éléments régulateurs suivants: a) un promoteur de CMV ou des variants fonctionnels de celui-ci, b) un intron, c) un TPL ou des variants fonctionnels de celui-ci, d) des gènes VA ou des variants fonctionnels de ceux-ci et e) une séquence de polyadénylation d'hormone de croissance bovine ou des variants fonctionnels de celle-ci.

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