Rna export element and methods of use

Details Rna export element and methods of use Rna export element and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C07K 14/02 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12N 15/86 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) C12N 15/51 (2006.01)

Patent

CA 2304207

A cis-acting posttranscriptional regulatory element (PRE) useful for efficient RNA export of RNA is provided. The element, termed WPRE, is originally derived from woodchuck hepatitis virus. The invention also provides a method for enhancing the expression of transgenes by insertion of the WPRE nucleic acid sequences in operably linkage with the transgene.

L'invention concerne un élément régulateur (PRE) cis de post-transcription utile pour transporter l'ARN de façon efficace. Cet élément, désigné WPRE, provient du virus de l'hépatite de la marmotte. Elle concerne également un procédé servant à amplifier l'expression de transgènes par insertion des séquences d'acides nucléiques de WPRE en liaison opérationnelle avec le transgène.

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