Nucleic acid amplification using non-random primers

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2626977

The present invention provides methods for selectively amplifying a target population of nucleic acid molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides populations of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS: 1-933. These oligonucleotides can be used, for example, to prime the synthesis of cDNA molecules complementary to mRNA molecules isolated from mammalian blood without priming the synthesis of cDNA molecules complementary to globin mRNA, or ribosomal RNA molecules.

La présente invention concerne des méthodes d'amplification sélective d'une population cible de molécules d'acides nucléiques (par exemple, toutes les molécules d'ARNm exprimées dans un type de cellule, à l'exception de l'espèce d'ARNm la plus exprimée). Cette invention a aussi trait à des populations d'oligonucléotides comportant les séquences d'acides nucléiques établies dans SEQ ID NOS: 1-933. Ces oligonucléotides peuvent être utilisés, par exemple, pour amorcer la synthèse des molécules d'ADNc complémentaires aux molécules d'ARNm isolées du sang mammalien, sans amorçage de la synthèse de molécules d'ADNc complémentaires à l'ARNm de globine ou aux molécules d'ARN ribosomique.

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