Elucidation of gene function

Details Elucidation of gene function Elucidation of gene function

C - Chemistry, Metallurgy

40

B

C40B 40/02 (2006.01) C12N 11/00 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01) C40B 30/00 (2006.01) C40B 40/06 (2006.01) C40B 50/06 (2006.01) C40B 50/18 (2006.01) C40B 60/00 (2006.01) C12N 15/87 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2445884

Articles and methods are provided for determining the function of genes in a rapid and cost effective manner. Nucleic acids are arrayed upon a substrate. In accordance with certain preferred embodiments, viable cells are subsequently caused to be bound to the substrate at the locations occupied by the nucleic acids. Subsequent transduction or transfection of the cells by the nucleic acids followed by continued vitality of the cells permits expression of the proteins encoded by the respected nucleic acids. Knowledge of the identity of the nucleic acids, at least as regards their locations on the substrate, permits determination of protein function thereof. Methods of creating and using such cell-arrays, and methods of reverse-transfection and reverse-transduction are featured.

Cette invention concerne des articles et des procédés qui permettent de déterminer rapidement et efficacement la fonction de gènes. Des acides nucléiques sont disposés sur un substrat. Selon certains modes de réalisation préférés, on relie ensuite des cellules viables au substrat au niveau des points occupés par les acides nucléiques. Une transduction ou une transfection ultérieure des cellules par les acides nucléiques sans perte de vitalité des cellules permet d'exprimer les protéines codées par les acides nucléiques respectifs. Connaissant l'identité des acides nucléiques, du moins d'après leurs emplacements sur le substrat, il est possible de déterminer la fonction protéique correspondante. Sont décrits des procédés permettant de créer et d'utiliser de tels ensembles de cellules ainsi que des procédés de transfection et de transduction inverses.

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