Method and device for cutting fresh tissue slices

Details Method and device for cutting fresh tissue slices Method and device for cutting fresh tissue slices

B - Operations, Transporting

26

D

B26D 7/08 (2006.01) B26D 1/08 (2006.01) G01N 1/28 (2006.01) G01N 1/36 (2006.01)

Patent

CA 2661866

Fresh tissue slices can be cut from tissue specimens embedded in a low gel point agarose by the use of a vibrating, sliding-blade microtome. The microtome has a blade which vibrates in a generally horizontal direction and which is supported for movement in a generally diagonal, sliding direction with respect to the tissue specimen. The compound vibrating and sliding motion of the cutting blade of the microtome is very effective in cutting fresh tissue slices that have a slice viability, measured as a ratio of live to dead cells, which is much better than that which has been obtained using conventional vibrating microtomes.

Des tranches de tissus frais peuvent être découpées à partir d'échantillons de tissus enrobés dans de l'agarose à bas point de gélification, au moyen d'un microtome à lame coulissante vibrante. Le microtome présente une lame apte à vibrer suivant une direction généralement horizontale, et qui est supportée de manière à coulisser, suivant une direction généralement en diagonale, par rapport à l'échantillon de tissu. Le mouvement composé, vibrant et coulissant, de la lame de coupe du microtome est très efficace pour la découpe de tranches de tissus frais présentant une viabilité des tranches, laquelle est mesurée en tant que rapport cellules vivantes/cellules mortes, ce rapport étant bien meilleur que celui obtenu au moyen de microtomes vibrants conventionnels

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