Diagnostics and vaccines for mycrobacterium paratuberculosis...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/04 (2006.01) A61P 31/04 (2006.01) C07K 14/35 (2006.01) C07K 16/12 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2473226

The present invention relates to nucleic acid sequences encoding Mycobacterium avium subspecies paratuberculosis proteins, to parts of such nucleic acid sequences that encode an immunogenic fragment of such proteins, to DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof. The invention also relates to Mycobacterium avium subspecies paratuberculosis proteins and immunogenic parts thereof encoded by such sequences. Furthermore, the present invention relates to vaccines comprising such nucleic acid sequences and parts thereof, DNA fragments, recombinant DNA molecules, live recombinant carriers and host cells comprising such nucleic acid sequences or such parts thereof, proteins or immunogenic parts thereof and antibodies against such proteins or immunogenic parts thereof. Also, the invention relates to the use of said proteins in vaccines and for the manufacture of vaccines. Moreover, the invention relates to the use of said nucleic acid sequences, proteins or antibodies for diagnostic or vaccination purposes. Also, the invention relates to methods for the preparation of such vaccines. Finally the invention relates to diagnostic kits comprising such nucleic acids, proteins or antibodies against such proteins.

L'invention concerne des séquences d'acide nucléique codant des protéines de la sous-espèce <i>paratuberculosis</i> de <i>Mycobacterium avium</i>, des parties de ces séquences d'acide nucléique codant un fragment immunogène de ces protéines, des fragments d'ADN, des molécules de recombinaison d'ADN, des vecteurs de recombinaison vivants et des cellules hôtes comprenant lesdites séquences d'acide nucléique ou leurs dites parties. L'invention concerne également des protéines de la sous-espèce <i>paratuberculosis</i> de <i>Mycobacterium avium</i> et des parties immunogènes de celle-ci codées par de telles séquences. En outre, l'invention concerne des vaccins comprenant des séquences d'acide nucléique et des parties de celles-ci, des fragments d'ADN, des molécules de recombinaison d'ADN, des vecteurs de recombinaison vivants et des cellules hôtes comprenant lesdites séquences d'acide nucléique ou leurs dites parties, leurs protéines ou parties immunogènes et des anticorps contres ces protéines ou leurs parties immunogènes. L'invention concerne enfin l'utilisation de ces protéines dans des vaccins et dans la fabrication de vaccins, l'utilisation des séquences d'acide nucléique, les protéine ou des anticorps susmentionnés dans le diagnostic ou la vaccination, des procédés de préparation de tels vaccins, et des nécessaires de diagnostic comprenant ces acides nucléiques, ces protéines ou des anticorps conte ces protéines.

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