G - Physics – 01 – N
Patent
G - Physics
01
N
167/43
G01N 33/48 (2006.01)
Patent
CA 1128417
INSOLUBILIZED DEOXYRIBONUCLEIC ACID (DNA) ABSTRACT OF THE DISCLOSURE A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immuno- fluorescent assay for such detection is described. The method comprises incubating an insoluble conjugate of methylated bovine serum albumin (mBSA)-native deoxyribonucleic acid (n-DNA) or other substrates with serum from patients with systemic lupus erythematosus (SLE) or other autoimmune diseases for d sufficient length of time, washing the pre- cipitate or flocculate, separating and discarding therefrom the super- natant fluid, adding a fluorescein labeled anti-immunoglobulin antibody to the washed flocculate and incubating the labeled mixture for a suf- ficient length of time, washing the incubated flocculate and separating therefrom the supernatdnt fluid, suspending the pellet in a liquid medium to determine the fluorescence of the flocculate, which is proportional to the concentration of antibodies to deoxyribonucleic acid and others in serum specimen. The insoluble conjugate of methylated bovine serum albumin (mBSA)-native deoxyribonucleic acid (n-DNA) retains the double- stranded form of native deoxyribonucleic acid (n-DNA).
388887
American Hospital Supply Corporation
Macrae & Co.
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